NEWSLETTER No 55 November 2000 

Archives

THIS MONTH

Dermatophytosis
Veterinary Nurse refresher courses
Next Clin Path Club meeting
What was it? answer
Rabies Virus in UK
Interesting facts about Rabbits
FAQ’s1. Rats as pets 2. Guinea pigs and vitamin C
Tail End: Rabbits play nurse maid to ostriches

Christmas and New Year Postal arrangements
As always from the beginning of December the postal service starts to slow down. To reduce the seasonal effect, post early in the day. Use Post Office Special Delivery for time sensitive samples or request a courier collection. Separate serum or plasma where possible and send fresh smears with your haematology samples. Pack samples in accordance with the Post Office regulations. For further information or assistance please contact Joanne Kenyon - Client Services

Christmas and New Year Holidays
The laboratory will be closed on the following dates and times:-
Monday 25th December CLOSED
Tuesday 26th December CLOSED
Monday 1st January CLOSED

Overnight Courier 
Last collection Friday 22nd December

Same Day Courier
Normal collections up to Friday 22nd December 
Normal collections from Wednesday 27th December
Please book collections as early as possible.

May we take this opportunity to say thank you for using NWL in 2000 we wish you a very Merry Christmas and a Peaceful and Prosperous New Year

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DERMATOPHYTOSIS 
Fungal infections represent 2% of all skin diseases
According to the literature skin disease resulting from dermatophyte infection represents about 2% of all dermatological cases. Dermatophytes are transmitted by direct contact or by contact with infected hair and scale in the environment or on fomites. One study found that infected hair in the environment could remain infective for eighteen months. The most commonly isolated dermatophyte in this laboratory is Microsporum canis. In cats the lesions vary from irregular patches of scale or alopecia to classic circular patches of alopecia with scaling, miliary dermatitis, onychomycosis and granulomatous dermatitis. Long haired cats may harbour the infection asymptomatically. 

Dermatophytosis should be considered as a differential in any papular or pustular eruption in dogs, which often develop classic foci of alopecia with follicular papules, pustules, scales or crusts. Facial folliculitis and furunculosis almost mimicking an autoimmune skin disease can develop, classically involving a Trichophyton mentagrophytes or T.erinaceae infection after the dog has been burrowing into hedge bottoms after rodents or hedgehogs. Kerion is apparently a common presenting sign of Microsporum gypseum infection.
In horses, Microsporum and Trichophyton infections can occur following direct contact or indirect via fomites. Environmental fungi may complicate the picture as they are often found on equine skin.

Dermatophytosis should never be diagnosed without accompanying confirmatory tests and these include direct microscopy, examination for fluorescence (which is limited to about 50% of M canis infections only), and fungal culture. In selecting a sample of hair for microscopy and culture it is best to pluck hairs or obtain them by ”tooth brushings” avoiding obviously inflamed areas or already broken hairs. The use of the toothbrush is the best way to sample an asymptomatic animal. Send us the brushings and the toothbrush in a sealed envelope. Surgical biopsy is another tool that can be used and is particularly useful where claws are involved.

We do not routinely undertake sensitivity testing of dermatophytes (although this is available if required) but currently preferred treatments are griseofulvin and itraconazole. The latter is regarded as superior for treating cats and dogs. 

Code DMC
Test Name Dermatophyte microscopic examination and culture
Samples Plucked hair and scrape
Cost £12.50 + vat

Code DC
Test Name Dermatophyte culture 
Sample Plucked hair
Cost £9.50 + vat

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PETS and Babesia
Sampling for blood born parasites
Since the introduction of the pets travel scheme (PETS) there have been a number of reported cases of Babesiosis in animals returning from Europe. Babesia can be diagnosed from the examination of a stained blood film.
As Babesiosis is a relatively rare diagnosis in the UK it is important to consider a number of points when submitting samples from animals with a history of travel to Europe. The history of recent travel to Europe should be indicated on the submission form. Blood smears should be made from blood taken from an ear margin. 
Dependent upon the stage of infection the number of organisms in circulation may be quite low. It has been noted that due to a sludging effect on infected RBC’s in the peripheral capillaries the organism is more likely to be seen in blood samples taken from an ear margin. 
Reference: Perkins S.C.B. (2000) Babesia and the Pet Travel Scheme. Veterinary Record 147 460
.
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CLIN PATH CLUB

Next Meeting: Thursday 11th January 2001

Venue: Myerscough College, Bowland Suite, Bilsborough, Lancashire

Programme: Imported Diseases and PETS – Illustrated talk

Case book studies: Cases wanted

To book your place, request further information or a location map call Joanne Kenyon on 01253 899215 or visit the web site - www.nwlabs.co.uk

CLIN PATH CLUB - Dates for 2001
There will be meetings on the following dates:-

Thurs 15th March
Thurs 10th May
Thurs 19th July
Thurs 20th September
Thurs 15th November
CLIN PATH CLUB Meeting 2nd November
The September meeting had to be cancelled because of the petrol shortage. The November meeting was nearly cancelled for the same reason. Tell people there is not going to be a petrol shortage and what happens everyone immediately fills up with petrol and creates a petrol shortage. A self fulfilling prophecy of ever there was one.
Of the 41 people who booked places only 19 managed to turn up which was a little disappointing. Jane Miller gave an illustrated talk on the Leukocyte and its role in diagnosis. Neil Roche a practitioner from Preston presented an interesting case study. This was followed by a demonstration of how to make a blood smear by Alistair Parker. A number of people took the opportunity to try smear making for themselves and a few shy ones would have liked to.

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NEW PRICE LIST
A new price list will be issued in the next few weeks and will become effective from 1st February 2001. 
In response to the feedback from last years price list we have included an indication of turnaround times. There are some areas where turnaround times can only be approximate. This applies particularly to microbiology where times can be dependant on growth rates, mix of organisms recovered, purity of isolations. We feel it is more important to get a correct result than push through samples to a strict time table. For none routine submissions or time critical samples we ask that you contact the laboratory for an accurate indication of turnaround times. 

The microbiology section has been completely revised. You will note that test options are now based on the nature of the sample submitted. This will enable us to reduce the cost of microbiological investigations in most cases and to provide an indication of the tests involved for a particular request. These changes are based on an analysis of the submissions over the past 12 months. There have regrettably been some price adjustments, on average these amount to approx 2%. 

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Chlamydophila

Name change for Chlamydia psittaci.
The taxonomy of the genus Chlamydiaceae has been revised into two genus:- Genus - Chlamydophila which contains the species C.psittaci, C.pneumonia, C.pecorum, C.felis, C.caviae, C.abortus
and 
Genus - Chlamydia which contains the species C.trachomatis, C,suis, C.muridarum. 

The disease is now called Chlamydiosis.

Reference:  Int J Syst Bacteriol 1999 49, 415-40

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FAQ’s
Is it helpful to submit smears when requesting haematology?

Yes. Blood cell morphology deteriorates from the moment the sample is drawn. By making smears from the freshly drawn blood the morphology of erythrocytes, leucocytes and platelets is preserved. If you can make the smears from blood before mixing with the EDTA anticoagulant so much the better. This is important if you are interested in Haemobartonella because EDTA can detach the parasite from the surface of the erythrocyte making detection much more difficult. For good cell preservation it is important to dry your smears quickly by waving about, do not leave them lying on the bench to dry, this induces artefacts.
IMPORTANT Do not put blood smears in the same pack as samples fixed in Formalin. Formalin, even contact with the fumes, prevents staining with the Romaowsky stains used on blood smears.

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Respiratory Disease in Rabbits

Pasteurella multocida is the commonest cause of respiratory disease in rabbits
Bordetella bronchiseptica and Staphylococcal infections have been associated with respiratory tract infections in rabbits. But Pasteurella multocida is the commonest cause of respiratory disease. 

Clinical signs may vary: chronic snuffles, purulent conjunctivitis, localized abscesses and sudden deaths due to septicaemic pasteurellosis may be seen.

Transmission is by direct contact with animals shedding P. multocida from nasal or vaginal excretions. Suckling rabbits may be infected with Pasteurella multocida from carrier does within the first week of life. Contrary to popular belief aerosols do not appear to be an important means of spread. The principle cause for sub-clinical carriers developing symptoms of Pasteurellosis is stress. This maybe poor ventilation, high ammonia levels due to poor hygiene, cold/damp conditions, overstocking and introducing new animals. In pet rabbits, signs of Pasteurellosis are normally limited to snuffles and conjunctivitis. Nasal swabs and throat swabs may be used to recover the causative organism.

Affected animals should be treated with parenteral antibiotic. Tetracyclines or fluoroquinalones are recommended. Individual rabbits with conjunctivitis may be treated by flushing the naso-lacrimal ducts with saline and instilling antibiotic. Treatment may need to be lengthy up to 4 weeks. Following treatment animals may be re-sampled for evidence of infection, 3 negative samples at least 7 days apart is advised.

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Tail End

Christmas comes early for Sooty
A guinea pig called Sooty had a night to remember after escaping from his pen and tunnelling into a cage of 24 females. 

He romanced each of them in turn and was yesterday the proud father of 43 offspring. Staff at Little Friend's Farm in Pontypridd, South Wales, have now secured Sooty's pen - and begun looking for homes for the guinea pigs. Owner Carol Feehan,, said: "I'm sure a lot of men will be looking at Sooty with envy. 

"We knew that he had gone missing after wriggling through the bars of his cage. We looked for him everywhere but never thought of checking the pen where we keep 24 females. We did a head count and found 25 guinea pigs - Sooty was fast asleep in the corner. He was absolutely shattered. We put him back in his cage and he slept for two days."

For Guinea Pig information try: http://www.petnetcanada.com/small_animals/guinea-pigs.htm

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