Test Protocol Index
Test Protocol 15

FELINE HIGH-DOSE DEXAMETHASONE/ACTH STIMULATION TEST
This combined screening test appears to be helpful in the diagnosis of feline hyperadrenocorticism. It involves taking 3 blood samples over a four-hour period, and is reported to be less affected by non-adrenal illness.

PROTOCOL

1. Take a basal blood sample for coritsol estimation.

2. Inject 0.1mg/kg dexamethasone (Hi-Dose) i.v.

3. Collect a 2-hour post dexamethasone sample for cortisol estimation.

4. Immediately inject 0.125 mg of synthetic ACTH (Synacthen, Alliance Pharmaceuticals) i.v.

5. Collect a third blood sample for cortisol estimation 1 hour after the ACTH.

INTERPRETATION

Normal cats show at least 50% cortisol suppression after dexamethasone and a normal coritisol response after ACTH stimulation (up to 400 nmol/l).

Cats with HAC show little suppression after dexamethasone and in exaggerated response following ACTH.
Test Code Test Name & Description Sample
DXAC Feline High Dose Dexamethasone/ACTH Stimulation Test 3 x Serum or Plasma

REFERENCE

Bsava Manual of Small Animal Endocrinology (2nd Ed).

Version 1, 21.05.03
 
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